Altogen Labs CRO Services:
RNAi Library Screening: Genome-wide siRNA and microRNA libraries
In the pursuit of gene functionality, RNAi library screening has become a very important research tool used to study gene function and entire signaling pathways. RNAi screening has allowed researchers to study the functionality of genes in a cell, both individually and in combinations of up to thousands of genes at a time. This technology is vital to understanding normal biological processes and disease mechanisms. RNAi libraries are developed to identify specific genes involved in biological processes and diseases. Altogen Labs offers siRNA and miRNA library screening, primary (high-throughput) and secondary cell-based assay development, and validation.
One of the major problems faced in the RNAi library screening is the delivery of stable, functioning siRNA, shRNA or miRNA to target cells. At Altogen labs we have developed and validated highly efficient and optimized delivery systems for over 80 cell lines to provide high throughput shRNA, siRNA and miRNA library screening. Altogen Labs scientists bring years of extensive cell culture experience and expertise.
Custom RNAi services include: siRNA Transfection, RNAi cell-based library screening, siRNA/shRNA design, synthesis, in vitro validation, qRT-PCR test of target mRNA knockdown, development of RNAi cell lines, mRNA reduction in tissues, siRNA targeting, chemical modification, cell transfection optimization, siRNA and microRNA genome-wide library screening, RNAi assay development. Altogen Labs offers transient and stable RNAi transfection services based on client individual specifications.
Transient RNAi transfection services include: Design, synthesis, HPLC purification, and annealing of 5 different siRNA molecules targeting gene of interest. siRNAs are tested for gene silencing potency. Two out of five siRNAs are finally selected (siRNAs are guaranteed to provide at least 80% mRNA expression knockdown). Transfection conditions are developed for a cell line(s) of choice. Validation of gene silencing (RNAi) effects is verified by real-time qRT-PCR (mRNA expression level) or Western Blot analysis (protein expression level).
see also: In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi Gene Knockdown Cell Lines | RNAi Screen Pathway Analysis: siRNA and microRNA | RNAi Manufacturing and CRO Services | PCR Cloning: DNA Vector Construction (shRNA) | Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB | Genome-wide siRNA and microRNA High-throughput Screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA Liposome Encapsulation Service | Transient (siRNA) and Stable (shRNA) Transfection Services