RNAi Screen Pathway Analysis: siRNA and microRNA
RNA interference (RNAi) is powerful experimental method for specific silencing of genes, and is now an important technology for the elucidation of gene function, as well as gene involvement in specific biological processes. RNAi screening technology (high-throughput siRNA and microRNA library screens) facilitates the identification of novel genes and their regulatory functions, as well as discovery of a new gene signaling pathways.
Genome-wide library screening produces comprehensive set of genes involved in cell signaling pathways, and enable secondary screens for more detailed pathway analysis and characterization. With over 30,000 genes in the human genome, simultaneous RNAi library screening of several thousands genes requires robust statistical pipeline and pathway analysis.
siRNA and microRNA Libraries
Altogen Labs offers a number of siRNA and microRNA libraries (including genome-wide and pathway-specific), experimental high-throughput library screening, data analysis and pathway mapping for RNAi knockdown experiments, generating lists of relevant genes, and functional gene association. We use different statistical tests and a range of options for data normalization for identification of siRNAs and a significance analysis for association with biological processes involving target genes.
A number of relevant CRO services are also available: siRNA liposome encapsulation, ELISA and cell-based assay development, in vitro siRNA validation and in vivo siRNA testing (biodistribution, tissue-targeting, xenograft models).
Please let us know the technical details of your project, so that we can provide an accurate quote and timeline estimate (contact e-mail: firstname.lastname@example.org or call Altogen Labs technical support at 512-433-6177).
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi Gene Knockdown Cell Lines | RNAi Screen Pathway Analysis: siRNA and microRNA | RNAi Manufacturing and CRO Services | PCR Cloning: DNA Vector Construction (shRNA) | Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB | Genome-wide siRNA and microRNA High-throughput Screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA Liposome Encapsulation Service | Transient (siRNA) and Stable (shRNA) Transfection Services