ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
ELISA and Cell-based Assay Development
Enzyme-linked immunosorbent assays (ELISAs) are often used for quantification of antibody or antigen binding activity. They are used to convert the binding activity of a molecule to a measurable spectrophotometric response. Variations on this method can be used to test different types of ligand binding or measure other qualities. ELISAs are a powerful tool commonly used in industrial applications for research, diagnostics, and quality control.
Cell-based assays allow the examination of biochemical processes in situ. Altogen Labs can culture a wide variety of cells, allowing for study of their signaling, internal chemical processes, and reactions to stimuli. In addition, our expertise in cell-based assays allows for a range of applications including cytotoxic assays, metabolic assays and cellular mechanism analysis. Cell functions that can be clarified with these types of assays include receptor activation, receptor binding, cell signaling and ligand internalization. Measurements of these processes with cell-based assays convey a more rounded picture of how they occur within their natural environment.
Altogen Labs possesses extensive experience in creating unique and novel approaches to immune- and cell-based methods. Platforms and approaches include enzyme immunoassay (EIA), Bio-Plex kinase profiling assays, biomarker analysis, receptor binding, cell signaling, cytotoxicity, cell cycle assays, cell viability, and cell proliferation.
Altogen Labs can develop a cell-based assay to quantify the effect of a client’s proprietary compound by quantitation of specific biomarkers, cytokines, or phosphoproteins in cell culture supernatant or whole-cell lysates. Such technology allows researchers to apprehend the biological functions of complex systems and identify new targets of interest.
Custom ELISA kits and cell-based assays are designed to measure protein concentrations of interest and quantify the associated biological activity of target antigens. Customizable monoclonal and polyclonal antibodies produce high affinity, specificity and sensitivity to immune- and cell- based assays to identify given target antigens.
• Antibody Testing, Custom labeling, Biotinylation, Purification
• Monoclonal and Polyclonal Antibody Development
• ELISA Assay Development
• ELISA Platform Optimization
• ELISA Assay Format Modification
ELISA and Cell-Based Assay Sample Analysis
Altogen Labs can use a robust cell-based ELISA assay to analyze customer-provided samples. Standard ELISA service include testing activity of the compound of interest on a panel of 15 intracellular Ser/Thr kinases and 17 Tyr kinases, using phospho-specific antibodies by the determination of substrate-specific phosphorylation activity induced by kinase activation.
The activity of the following kinases can be profiled:
• Ser/Thr kinases: CDK2, IRAK4, GPKA, PKCβ, MAPK2, CDK4, JNK, PKB, p38α, MKK6, GS3Kβ, MAPK1, PKCα, p38β, IKK1
• Tyr Kinases: Abl, HGFR, Lck, Src, TrkC, EGF-R, IGF1-R, PDGFRα, Syk, VEGFR2, FIt-l IR, PDGFRβ, REC, Zap70, Jak2, RET, TRK
At Altogen Labs, we recognize that providing fast delivery of products and services is critical to expediting research. We are committed to meeting our clients’ timely needs while maintaining high quality and integrity. All of our services undergo rigorous QC procedures.
Once we know the details of your project, we can provide you an immediate price quote (contact us via e-mail at firstname.lastname@example.org or call Altogen Labs at 512-433-6177). Please note that experimental details will help us to provide an accurate quote and timeline estimate.
Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi Development: Targeted siRNA, qPCR Assays | Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting: Technology Development and Evaluation