Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines

Half maximal inhibitory concentration, or IC₅₀, is a measurement representing the halfway point in which a compound of interest produces complete inhibition of a biological or biochemical function. This information is derived based on pharmacological data in reference to a dose-response curve. As the dosage of an inhibitory compound is increased, the biological function it affects decreases, and the point at which the concentration of the inhibitor has suppressed 50% of the biological activity is referred to as the IC₅₀. IC₅₀is most often used as a measurement of antagonist, or inhibitory drug potency, as well as a quantification of the toxicological effects of inhibitory compounds.

During the development of Investigational New Drug (IND) applications, in vitro and in vivo toxicology testing studies are important to determine the threshold of inhibition of biological functions and effects of the compound on metabolism. In an effort to evaluate new pharmaceutical drugs and therapeutic treatments, cancer cell lines are used as in vitro models to screen the candidate drugs. Altogen Labs maintains a large library of cell lines that can be used for in vitro IC₅₀ studies (see Table 1 below for some selected examples) and has the capacity to utilize client-provided cell lines as well.

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Pharmacology & Toxicology Testing:
IC50 for Tumor Cell Lines

Table 1. Selected cancer cell lines available at Altogen Labs for in vitro IC50 testing:

Disease / Tumor Type:	Cell Line / ID
Breast Adenocarcinoma	SKBR3 (HTB30), MDA-MB (HTB26), MCF7 (HTB22)
Burkitt’s Lymphoma	Daudi (CCL-213), Raji (CCL-86), Ramos (CRL-1596)
Colon Carcinoma	Caco2 (HTB37), LS174T (CL188), SW480 (CCL228)
Lung Adenocarcinoma	NCI-H460 (HTB-177), NCI-H23 (CRL-5800)
Leukemia	K-562 (CCL-243), CCRF-CEM (CCL-119)
Lewis Lung Carcinoma	Calu-3 (HTB-55), Calu-6 (HTB-56), A549 (CCL-185)
Kidney	MDCK (CCL34), Cos7 (CRL1651), HEK293 (CRL1573)
Melanoma	J774A.1 (TIB-67), SK-MEL-28 (HTB-72)
Neuroblastoma	SK-N-SH (HTB-11), Neuro-2a (CCL-131)
Osteosarcoma	MG-63 (CRL-1427), SK-OV-3 (HTB-77)
Ovarian Adenocarcinoma	Beta-TC-6 (CRL-11506), CHO-K1 (CRL-9618)
Pancreatic Carcinoma	Capan-1 (HTB-79), MIA PaCa-2 (CRL-1420)
Prostate Carcinoma	LNCaP (CRL-1740), DU145 (HTB-81)
Rhabdomyosarcoma	Detroit 551 (CCL-110)

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Once we know the details of your project, we can provide you an immediate price quote (contact us via e-mail at info@altogenlabs.com or call Altogen Labs at 512-433-6177). Please note that experimental details will help us to provide an accurate quote and timeline estimate.