Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines

Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines

Half maximal inhibitory concentration, or IC50, is a measurement representing the halfway point in which a compound of interest produces complete inhibition of a biological or biochemical function. This information is derived based on pharmacological data in reference to a dose-response curve. As the dosage of an inhibitory compound is increased, the biological function it affects decreases, and the point at which the concentration of the inhibitor has suppressed 50% of the biological activity is referred to as the IC50. IC50 is most often used as a measurement of antagonist, or inhibitory drug potency, as well as a quantification of the toxicological effects of inhibitory compounds.

During the development of Investigational New Drug (IND) applications, in vitro and in vivo toxicology testing studies are important to determine the threshold of inhibition of biological functions and effects of the compound on metabolism. In an effort to evaluate new pharmaceutical drugs and therapeutic treatments, cancer cell lines are used as in vitro models to screen the candidate drugs. Altogen Labs maintains a large library of cell lines that can be used for in vitro IC50 studies (see Table 1 below for some selected examples) and has the capacity to utilize client-provided cell lines as well.

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Table 1. Selected cancer cell lines available at Altogen Labs for in vitro IC50 testing:

Disease / Tumor Type: Cell Line / ID
Breast Adenocarcinoma SKBR3 (HTB30), MDA-MB (HTB26), MCF7 (HTB22)
Burkitt’s Lymphoma Daudi (CCL-213), Raji (CCL-86), Ramos (CRL-1596)
Colon Carcinoma Caco2 (HTB37), LS174T (CL188), SW480 (CCL228)
Lung Adenocarcinoma NCI-H460 (HTB-177), NCI-H23 (CRL-5800)
Leukemia K-562 (CCL-243), CCRF-CEM (CCL-119)
Lewis Lung Carcinoma Calu-3 (HTB-55), Calu-6 (HTB-56), A549 (CCL-185)
Kidney MDCK (CCL34), Cos7 (CRL1651), HEK293 (CRL1573)
Melanoma J774A.1 (TIB-67), SK-MEL-28 (HTB-72)
Neuroblastoma SK-N-SH (HTB-11), Neuro-2a (CCL-131)
Osteosarcoma MG-63 (CRL-1427), SK-OV-3 (HTB-77)
Ovarian Adenocarcinoma Beta-TC-6 (CRL-11506), CHO-K1 (CRL-9618)
Pancreatic Carcinoma Capan-1 (HTB-79), MIA PaCa-2 (CRL-1420)
Prostate Carcinoma LNCaP (CRL-1740), DU145 (HTB-81)
Rhabdomyosarcoma Detroit 551 (CCL-110)

At Altogen Labs, we recognize that providing fast delivery of products and services is critical to expediting research. We are committed to meeting our clients’ timely needs while maintaining high quality and integrity. All of our services undergo rigorous QC procedures.

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Once we know the details of your project, we can provide you an immediate price quote (contact us via e-mail at info@altogenlabs.com or call Altogen Labs at 512-433-6177). Please note that experimental details will help us to provide an accurate quote and timeline estimate.

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IC50 for Tumor Cell Lines

GLP Compliant

Complete list of cancer cell lines available at Altogen Labs for IC-50 study:

293T Cells  (CRL-11268)
3LL  (Lewis Lung Carcinoma)
3T3-L1  (CL-173)
4T1  (Breast Cancer, CRL-2539)
786-O Cells  (CRL-1932)
A204  (Rhabdomyosarcoma)
A375 Cells  (CRL-1619)
A431  (Epidermoid Carcinoma)
A549  (Lung Carcinoma, CCL-185)
AGS  (Gastric Adenocarcinoma)
AR42J  (Pancreatic Carcinoma)
ARPE-19  (Retinal Pigment Epithelium)
ASMC  (Aortic Smooth Muscle Cells)
AsPC-1  (Pancreatic Beta Cells)
Astrocyte 
B16-F10  (Mouse Melanoma Cells)
BALB/3T3  (Embryonic Fibroblasts)
bEnd-3  (Brain Endothelioma)
Beta-TC-6  (Pancreas Tumor, CRL-11506)
BHK-21  (Kidney Cells, CCL-10)
BJ  (Skin Fibroblast Cells)
BMS2  (Bone Marrow Cells)
BT-20  (Breast Carcinoma Cells)
BxPC-3  (Pancreatic Cancer Cells)
C2C12  (Mouse Myoblast Cells)
C6  (Glioma Cells, CCL-107)
Caco-2  (Colon Carcinoma Cells)
Caki-1  (Kidney Epithelial Cells)
Calu-3  (Lung Carcinoma, HTB-55)
Calu-6  (Lung Carcinoma, HTB-56)
Capan-1  (Pancreatic Carcinoma)
CFPEo (HTRpC)  (Trachea Epithelium)
CHO  (Chinese Hamster Ovary Cells)
CHO-K1  (Ovarian Adenocarcinoma)
Chromaffin  (Chromaffin Cells)
CLBPEC  (Neuroblastoma Cells)
COLO205  (Colon Carcinoma)
COS-7  (Kidney Cells, CRL-1651)
CT26.WT  (Colon Carcinoma)
D-407  (Retina Pigment Epithelial)
D-451  (Mouse Fibroblast Cells)
Detroit551  (Rhabdomyosarcoma Cells)
DI-TNC1  (Rat Brain Astrocytes)
DLD-1  (Colon Cancer, CCL-221)
DU145  (Prostate Carcinoma Cells)
FaDu  (Hypopharyngeal Carcinoma)
Fibroblast  (Fibroblast Cells)
H9c2  (Embryonic Myocardium)
HCAEC  (Coronary Artery Endothelial)
HCC1937  (Breast Carcinoma)
HCN-1A  (Brain Neuronal Cells)
HCS-2  (Chondrosarcoma Cells)
HCT-116  (Colon Carcinoma)
HEK-293  (Epithelial Kidney Cells)
HeLa  (Cervix Adenocarcinoma)
HeLa-S3  (Cervical Adenocarcinoma)
Hep-3B  (Hepatocellular Carcinoma)
Hepa 1-6  (Hepatoma Cells)
HepG2  (Hepatocellular Carcinoma)
HMEC  (Mammary Epithelial Cells)
HT-1080  (Fibrosarcoma Cells)
HT-29  (Colorectal Adenocarcinoma)
HUH-7  (Liver Cancer Cells)
HUVEC , Umbilical Vein Endothelial Cell
IMR-90  (Lung Fibroblast, CCL186)
IRR-MRC5  (Irradiated Fibroblasts)
J774A  (Melanoma Cells, TIB-67)
KB  (Mouth Epidermal Carcinoma)
Keratinocyte  (Keratinocytes)
L6  (Myeloma Cells, CRL-1458)
LNCAP  (Prostate Carcinoma)
LoVo  (Colon Adenocarcinoma)
LS-174T  (Colon Adenocarcinoma)
MCF-7  (Breast Cancer, HTB-22)
MDA-MB  (Breast Adenocarcinoma)
MDCK  (Kidney Cells, CCL-34)
MEF  (Mouse Fibroblast Cells)
MG-63  (Osteosarcoma, CRL-1427)
MIA PaCa-2  (Pancreatic Carcinoma)
MRC5  (Diploid Fibroblast Cells)
NCI-H1299  (Lung Adenocarcinoma)
NCI-H1975  (Lung Carcinoma)
NCI-H23  (Lung Adenocarcinoma)
NCI-H292  (Lung Carcinoma, CRL1848)
NCI-H358  (Bronchioalveolar Cells)
NCI-H441  (Lung Carcinoma)
NCI-H460  (Lung Carcinoma, HTB177)
NCI-N87  (Gastric Carc. CRL-5822)
Neuro-2a  (Neuroblastoma, CCL-131)
NIH3T3  (Swiss Mouse Fibroblasts)
NTERA-2  (Embryonal Carcinoma)
PANC1  (Non-endocrine Pancreatic Cn)
PC-12  (Rat Pheochromocytoma Cells)
PC-3  (Prostate Cancer Cells)
PGHAM  (Pancreatic Carcinoma)
RD  (Retinal Epithelium Cells, CCL136)
SK-BR3  (Breast Adenocarcinoma)
SK-MEL-28  (Melanoma Cells, HTB72)
SKNAS  (Neuroblastoma Cells)
SK-N-MC  (Neuroblastoma Cells)
SK-N-SH  (Neuroblastoma, HTB-11)
SK-OV-3  (Human Osteosarcoma)
SW480  (Colon Carcinoma, CCL228)
T98G  (Glioblastoma Cells, CRL-1690)
U87  (Glioblastoma Cells)
VERO  (Monkey Kidney Cells)
Weri-Rb-1  (Retinoblastoma Cells)
ZR-75-1  (Breast Carcinoma, CRL1500)

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Pharmacology & Toxicology Testing:
IC50 for Tumor Cell Lines