siRNA Liposome Encapsulation Service
Liposome Encapsulation of Small Interfering RNA (siRNA) Molecules
Liposomes are composite structures made of phospholipids that areartificially made to encapsulate various types of nucleic acids within the aqueous core of the liposome. Due to the hydrophobic nature of the liposome outer membrane it can fuse with other lipid bilayers, such as the cell membrane, and deliver its content via cellular fusion.
Lipofection is the process in which liposomes are used for transfection of DNA into a host cell. Liposomes are used for the highly efficient delivery of cargo molecules (DNA, RNA, and protein) into cells both in vitro and in vivo. Liposomes containing charged molecules and drugs can effectively deliver otherwise impermeable compounds within the host cell. This technology is known to be very useful for intracellular delivery of drugs and associated research and development.
Altogen Labs offers encapsulation of negatively charged molecuIes (e.g., siRNAs, shRNAs, microRNAs, mRNAs, plasmid DNAs, and proteins) into either one of several standard liposome formulations (PC : Cholesterol and DSPC : Cationic Lipid : PEGylated Lipoid : Cholesterol) or a custom liposome formulation. Custom experimental design of liposome is available to provide in vivo siRNA protection (in the liposomal core), PEG-modifications to reduce the innate immune response, and low cytotoxicity using biodegradable lipids and polymers.
Sonication, liposome extrusion, and microfluidization (microfluidics) methods allow the generation of liposomes of a defined size (from 50 nm to 400 nm). The customer should provide at least 0.25 mg of siRNA for liposome encapsulation. Liposome-encapsulated siRNAs are delivered to the customer customer in sterile vial to ensure consistency and eliminate potential contamination.
Our experienced staff ensures high quality liposome products that are cost effective, reliable and consistent. At Altogen Labs we devote ourselves to excellence while providing quality ensured products for research and development. Altogen Labs previously developed a line of in vivo transfection products including PEG-liposomes and nanoparticle-, polymer- and lipid-based in vivo transfection systems for laboratory testing of pharmaceuticals in cells and animals.
Once we know the details of your project, we can provide you an immediate price quote (contact e-mail: firstname.lastname@example.org). Please note that experimental details will help us to provide an accurate quote and timeline estimate.
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi Gene Knockdown Cell Lines | RNAi Screen Pathway Analysis: siRNA and microRNA | RNAi Manufacturing and CRO Services | PCR Cloning: DNA Vector Construction (shRNA) | Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB | Genome-wide siRNA and microRNA High-throughput Screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA Liposome Encapsulation Service | Transient (siRNA) and Stable (shRNA) Transfection Services