Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- BACTERIA ID 16S SEQUENCING
Cryopreservation and Cell Banking Service (GLP-compliant)
Altogen GLP-Compliant Cell Banking and Cryopreservation Service
Cryogenic storage at ultralow temperatures is a recognized mechanism for providing indefinite longevity to cells; however researchers have observed variability in the preservation of cells when samples were kept at different frozen temperatures. Generally, liquid nitrogen at -196°C is required to successfully preserve the complex biological structures and to virtually stop all biological activity.
Altogen Labs complies with regulatory guidelines to ensure that all procedures, practices, and facilities conformto GLP standard. Our GLP-compliant cryopreservation and cell line banking systems provide mammalian cell bank production and all types of associated services. All cell lines are certified free of mycoplasma or bacterial contamination.
Our protocols use suitable combinations of cryoprotectants and cooling regimes to guarantee successful cryopreservation of biological materials, cells, and tissues including stable cell lines, cells for transfusion, umbilical cord blood cells, stem cells, and tumor/ histological cells.
Altogen Labs offers long-term cell storage of cells in a secure, controlled, and monitored environment. Cell banking work is performed within certified Class II laminar flow biosafety cabinets with HEPA filtered air. Cells are grown only in calibrated incubators with ultraviolet (UV) air and water protection. Banks are divided and stored in separate freezers to minimize the risks of a freezer malfunction. Liquid nitrogen levels and freezer temperatures are monitored 24 hours per day, 7 days per week with a special monitoring system. We ensure the security of the storage facility with a fully redundant backup system.
Cryovials can be used to expand a cell population, or cane be shipped directly to the client or to a specified third party. We ship cells on dry ice to any destination worldwide.
see also: Cryopreservation and Cell Banking Service (GLP-compliant) | Cell Banking Service: Master Cell Bank (MCB, WCB) Generation