Development of Stable Cell Lines
Stable cell lines expressing a protein of interest (or shRNA constructs targeting gene of interest) are important research tools in life science, widely used in drug discovery and compound screening programs. Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected plasmid DNA or reporter gene constructs into the genome. The development of stable cell lines is an important research tool in pharmaceutical drug discovery, compound screening and gene therapy research. Development and validation of stable cell lines can be risky, expensive, and time- and labor-consuming.
Altogen Labs has unique experience with more than 150 cancer cell lines, customizable reporter systems, highly efficient gene delivery technologies, as well as the research expertise to produce stably expressing clonal cells in just 28 days (read more about Generation of Stable Cell Lines in 28 Days Service). Company scientists bring years of cell culture experience and expertise to this service. Experimental protocols include transfection of plasmid DNA, drug selection of clonal cells, colony pickup, functional and expression screening, generation of stable lines, validation of construct expression by real-time qRT-PCR, and Western blot analysis.
Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected plasmid DNA or reporter gene constructs into the genome. The development of stable cell lines is an important research tool in pharmaceutical drug discovery, compound screening and gene therapy research.
Altogen Labs offers several stable cell line generation services:
– Reporter stable cell lines (luciferase, GFP, RFP, YFP)
– Protein overexpressing cell lines
– Knockdown (RNAi) cell lines
Our scientists can transform your cell line to stably express a protein of interest, or a shRNA-encoding plasmid targeting gene of interest. We perform clonal cell line selection and cell population expansion to develop a cell line that expresses specific genes integrated into the cellular genome.
Altogen Labs provides innovative life science research services, including RNAi services, generation of stable cell lines, compound encapsulation services, and many other biology contract research services. Altogen Labs is committed to offering the highest quality service at the lowest price for scientific research.
At Altogen Labs, we recognize that providing fast delivery of products and services is critical to expediting research. We are committed to meeting our clients’ timely needs while maintaining high quality and integrity. All of our services undergo rigorous QC procedures.
Once we know the details of your project, we can provide you an immediate price quote (contact us via e-mail at firstname.lastname@example.org or call Altogen Labs at 512-433-6177). Please note that experimental details will help us to provide an accurate quote and timeline estimate.