Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
Caspase-3/7 activity cell based assay is standard research tool that allows to quantitate apoptosis induction in tissue specific biological model. Customer sends compound of interest, and Altogen Labs scientists perform an apoptosis cell-based assay (over 120 cancer cell lines are available in-house).
The relative amount of caspase per cell is estimated using luminogenic caspase-3 and caspase-7 substrates in a buffer system optimized for caspase activity, luciferase activity, and cell lysis. Cells in logarithmic growth are seeded in 96-well plates in 0.2 mL media. Cell lines are cultured with a single concentration of compound of interest (dose-response study). Cells are harvested at 6, 12, 24, and 48 hr after addition of compound.
The caspase reagent is added to the cells in the well, resulting in cell lysis, followed by caspase cleavage of the substrate and generation of a “glow-type” fluorescent signal. A fluorescence intensity is measured using Molecular Device Spectramax Gemini XPS plate reader and the signal generated is proportional to the amount of caspase activity present.
Once we know the details of your project, we can provide you an immediate price quote (contact e-mail: email@example.com or call Altogen Labs technical support at 512-433-6177). Please note that experimental details will help us to provide an accurate quote and timeline estimate.
Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi Development: Targeted siRNA, qPCR Assays | Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting: Technology Development and Evaluation