Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- BACTERIA ID 16S SEQUENCING
Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
Liposomal encapsulation of siRNA, shRNA, microRNA, mRNA, protein, and plasmid DNA molecules:
Liposomes are composite phospholipid structures that can be made to encapsulate various types of molecules within the aqueous core of the liposome. Liposomes are used to efficiently deliver cargo molecules (such as siRNA, mRNA, DNA, RNA, protein) into cells in vitro and in vivo.
Altogen Labs offers encapsulation of charged molecuIes (small molecules, siRNA, shRNA, microRNA, mRNA, plasmid DNA, protein) into one of the standard liposomes (PC:Cholesterol and DSPC:Cationic Lipid: PEGylated Lipoid:Cholesterol) or into a custom liposome formulation.
Sonication and liposome extrusion methods allow Altogen Labs to generate liposomes of defined size (from 50- to 400-nm particles). Clients should provide at least 0.1 mg of cargo molecule (f.e. siRNA) for liposome encapsulation.
Altogen Labs offers pioneering life science research services, including a number of of pre-clinical studies, microorganism identification, development of stable cell lines, in vivo siRNA delivery and complete A-to-Z RNAi gene silencing services: gene targeting, siRNA synthesis, functional in vitro validation, siRNA encapsulation, in vivo siRNA protection and tissue targeted delivery. Altogen Labs offers the highest quality CRO service at the lowest price for scientific research for pharmaceutical and biotechnology industry and academic institutions.
Our quotes for price and timeline are based on your project’s unique experimental details.
Please contact us for an immediate price and timeline estimate at info@altogenlabs.com
see also: Pharmacology and toxicology testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi development: targeted siRNA, qPCR assays | Liposome Encapsulation services: siRNA, miRNA, DNA, proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting Services: technology delevopment & evaluation