Bacterial Analysis: 16S Sequencing

Bacterial Analysis: 16S Sequencing

Genotyping applications largely depend on sequencing data (often 16S and 5S). Pyrosequencing can establish precise genetic sequence information because this technology sequences by synthesizing new copies of DNA template, and the results provide unambiguous data.

Pyrosequencing technology reliably reports the actual sequence of the locus. Thus, a single assay can detect a range of possible mutations including new and unexpected changes. Altogen Labs performs extraction of genomic DNA, performs bisulfite conversion of genomic DNA, optimizes and performs biotinylated PCR, performs Pyrosequencing reaction, and produces analyzed results.

Pyrosequencing allows identification of a large number of species using a single conserved sequencing primer.

Depending on experimental assay design, pyrosequencing can be used to discriminate microbial species, types, and strains, or detect genetic mutations that confer resistance to antibiotics or antiviral drugs.

A growing number of pyrosequencing assays specifically target commonly studied pathogenic species and strains of bacteria, viruses, and fungi.

Service Options:

Pyrosequencing reaction only:

Customer provides PCR amplicon and Pyrosequencing primer

Altogen Labs performs pyrosequencing reaction and produces analyzed results

PCR + Pyrosequencing reaction:

Customer provides DNA template, PCR amplicions, and Pyrosequencing primer

Altogen Labs optimizes and performs biotinylated PCR, performs Pyrosequencing reaction, and produces analyzed results

Extraction + PCR + Pyrosequencing reaction:

Customer provides cells, tissue, biopsy, etc…

Altogen Labs performs extraction of genomic DNA, optimizes and performs biotinylated PCR, perform Pyrosequencing reaction, and analyze results

Extraction + Bisulfite Conversion + PCR + Pyrosequencing reaction:

Customer provides cells, tissue, biopsy, etc…

Altogen Labs performs extraction of genomic DNA, performs bisulfite conversion of genomic DNA, optimizes and performs biotinylated PCR, performs Pyrosequencing reaction, and produces analyzed results

Assay Design:

Customer provides the sequence

Altogen Labs designs Pyrosequencing assay

We can provide an immediate price quote (contact e-mail: info@altogenlabs.com). Please note that experimental details will help us provide an accurate quote and timeline estimate.

GLP Compliant


see also:
What is Microorganism Identification? Method Description | Scientific Background: Specific 16S rRNA Regions | Bacterial Analysis: 16S Sequencing | Microbial Identification Service: Applications