Stimulation of Adipogenesis (Gly)
Adipogenesis is the process of cell differentiation of pre-adipocytes (derived from mesenchymal stem cells (MSCs)) into adipocytes. Adipocytes are responsible for lipid storage of triglycerides and whole-body energy homeostasis in animals. Adipogenesis is tightly regulated by hormonal and growth factor signaling, stimulating pre-adipocyte differentiation and accumulation of lipids in the form of triglycerides.
Extensive research on adipogenesis has implicated dysfunctioning adipocytes in pathological states such as diabetes, atherosclerosis, cancer and cardiovascular disease. Further research can identify regulators of adipogenesis and potentially find ways to prevent and treat diabetes and other obesity related diseases.
Adipogenesis Enzymatic Assays:
Total triglyceride differentiation assays are performed in a 96-well or 384-well plate format using subcutaneous pre-adipocytes. Compounds are tested for their ability to increase triglyceride accumulation during the differentiation process compared to the positive control (complete differentiation medium).
Triglycerides are quantified using a coupled enzyme-colorimetric reaction and compared to a standard curve. A DMSO vehicle is a negative control, and PPARα agonists are positive controls. This service includes 10 customer-defined compound concentrations, each tested in triplicate. The optimal sample for the service is a compound dissolved in PBS or culture medium.
Once we know the details of your project, we can provide you an immediate price quote (contact e-mail: firstname.lastname@example.org or call Altogen Labs technical support at 512-433-6177). Please note that experimental details will help us to provide an accurate quote and timeline estimate.