SAS xenograft model
Oral cancer is frequently diagnosed at a late stage, and the outlook for complete recovery and survival is not very optimistic. However, if diagnosed early, the five-year survival for oral cancer is 75 percent versus only 20 percent for patients with late-stage diagnosis, as stated by the U.S. Department of Health and Human Services. Improvements in treatment options for late-stage patients require extensive preclinical studies that involve human tumor xenograft models. The SAS cell line was isolated from a poorly differentiated squamous cell carcinoma of the tongue. A 2013 study by Li et al. published in International Journal of Oncology, examined the antitumor effects of the scutellarin on human tongue squamous carcinoma (SAS) cell line in vitro and in vivo, using the SAS xenograft model. Also, the molecular mechanism of scutellarin has been investigated. These findings report that the scutellarin treatment significantly inhibits the growth of xenograft SAS tumors in immunodeficient mice. Also, scutellarin regulates inhibition of tumor cell proliferation, induces apoptosis and mediates expression of MMP-2, MMP-9, and integrin αvβ6 at the mRNA and protein levels in vivo. The article demonstrates that scutellarin has an anti-tumor therapeutic effect by inhibition of the ability of SAS cells to metastasize. The SAS cell line (human tongue) is used to create the CDX (Cell Line Derived Xenograft) SAS xenograft mouse model. The SAS xenograft model exhibits anti-tumor activity after treatment with platinum-based chemotherapeutic agents, taxanes and valproic acid.
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Basic study design
- All flasks containing cells are maintained at a confluency allowing exponential growth. After trypsinization, cell viability and concentration is determined by trypan blue. Density is adjusted such that 1 x 10^6 cells in a 100 uL inoculation are inoculated (matrigel + SAS). Subcutaneously (s.c.), injections are performed into the hind leg of athymic BALB/C or NOD/SCID mice (10 to 12 weeks old).
- Treatment cohorts are formed after randomization of mice, which marks the beginning of the study. All test materials are injected following the client supplied dosage concentrations and schedules.
- All tumor measurements are logged (daily measurements), including mouse body weights (tri-weekly).
- The end of the study is reached at the tumor size limits (or 2,000 mm2), and necropsies are performed. Customer instructions determine the specific tissues to be collected and the method of storage (RNAlater, frozen, 10% NBF, etc). Tumors are weighed; optional digital imaging is available upon request.
Xenograft animal models are used to assess the effectiveness of drugs against specific types of cancer. New medicines are tested on staged tumor growths that have been engrafted via subcutaneous or orthotopic inoculation in an immunocompromised mouse or rat model. All clinically approved anti-cancer agents have been evaluated with conventional preclinical in vivo models. Xenograft studies can be highly complex, starting with the selection of the appropriate animal model, choice of tumorigenic cell line, administration method, dosing, analysis of tumor growth rates and tumor analysis (histology, mRNA and protein expression levels).
Altogen Labs provides an array of laboratory services using over 30 standard Cell Line Derived Xenograft (CDX) models and over 20 PDX models. Researchers investigating the role of specific proteins or gene products in regulating tumor growth can benefit from development of protein overexpression (genetically engineered to ectopically express proteins, tumor suppressors, or oncogenes) and RNAi cell lines with long term gene silencing. Altogen Labs provides quantitative gene expression analysis of mRNA expression (RT-PCR) and protein expression analysis using the WES system (ProteinSimple).
The dosing of the experimental compound of interest is initiated, for a staged study, when the mean tumor size reaches a specified volume (typically 50-100 mm3). In an unstaged study, the dosing of the compound of interest is initiated immediately after xenografting. Mice are dosed once or twice a day for 28 days (or other desired study duration) via the chosen route of administration. Tumor volume (mm3) is calculated via the “(W x W x L) / 2” formula, where W is tumor width and L is tumor length.
Animal handling and maintenance at the Altogen Labs facility is IACUC-regulated and GLP-compliant. Following acclimation to the vivarium environment, mice are sorted according to body mass. The animals are examined daily for tumor appearance and clinical signs. We provide detailed experimental procedures, health reports and data (all-inclusive report is provided to the client that includes methods, results, discussion and raw data along with statistical analysis). Additional services available include collection of tissue, histology, isolation of total protein or RNA and analysis of gene expression. Our animal facilities have the flexibility to use specialized food or water systems for inducible gene expression systems.
Following options are available for the SAS xenograft model:
- SAS Tumor Growth Delay (TGD; latency)
- SAS Tumor Growth Inhibition (TGI)
- Dosing frequency and duration of dose administration
- Dosing route (intravenous, intratracheal, continuous infusion, intraperitoneal, intratumoral, oral gavage, topical, intramuscular, subcutaneous, intranasal, using cutting-edge micro-injection techniques and pump-controlled IV injection)
- SAS tumor immunohistochemistry
- Alternative cell engraftment sites (orthotopic transplantation, tail vein injection and left ventricular injection for metastasis studies, injection into the mammary fat pad, intraperitoneal injection)
- Blood chemistry analysis
- Toxicity and survival (optional: performing a broad health observation program)
- Gross necropsies and histopathology
- Positive control group employing cyclophosphamide, at a dosage of 50 mg/kg administered by intramuscular injection to the control group daily for the study duration
- Lipid distribution and metabolic assays
- Imaging studies: Fluorescence-based whole body imaging, MRI