Altogen Labs CRO Services:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi Development: Targeted siRNA, qPCR Assays
- Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting: Technology Development and Evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi)
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi Gene Knockdown Cell Lines
- RNAi Screen Pathway Analysis: siRNA and microRNA
- RNAi Manufacturing and CRO Services
- PCR Cloning: DNA Vector Construction (shRNA)
- Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB
- Genome-wide siRNA and microRNA High-throughput Screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA Liposome Encapsulation Service
- Transient (siRNA) and Stable (shRNA) Transfection Services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- MICROORGANISM ID SERVICE
In Vivo Stable siRNA and microRNA: Chemical Modification
Altogen Labs developed methods to prevent siRNA degradation in serum, increase delivery efficiency to target organ, therefore providing strong functional effect (phenotype) induced by siRNA in vivo.
RNAi-induced gene silencing is used to study gene function in cultured cells, however this approach does not allow a critical evaluation of gene function within the whole organism. Unfortunately, application of RNAi in vivo may present many technical challenges due to siRNA stability, delivery efficiency, and tissue targeting.
Performing RNAi experiments in animals can be laborious, time-consuming, expensive, and present numerous technical challenges. Altogen Labs offer complete A-to-Z RNAi services, including in vivo siRNA development, chemical modification of siRNA (increase stability in serum), siRNA encapsulation into liposome, siRNA tissue targeting, qRT-PCR assay to measure siRNA accumulation, biodistribution, functional target mRNA knockdown, reduction of protein expression, off-target effects, immune system response, and many other RNAi in vivo services.
Altogen Labs provides complete in vivo siRNA development services, encapsulation, and tissue targeted delivery. Multiple siRNA administration routes, local and systemic: Intravenous, Intraperitoneal, Intranasal, Intratumoral, Intratracheal, Intradermal, Intramuscular, Intrathecal, Intracerebellar, Intravascular perfusion.
see also: In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi Gene Knockdown Cell Lines | RNAi Screen Pathway Analysis: siRNA and microRNA | RNAi Manufacturing and CRO Services | PCR Cloning: DNA Vector Construction (shRNA) | Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB | Genome-wide siRNA and microRNA High-throughput Screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA Liposome Encapsulation Service | Transient (siRNA) and Stable (shRNA) Transfection Services