Altogen Labs CRO Services:
Altogen Labs provides RNAi reporter assay and RNAi cell line development services.
RNAi stable cell lines expressing shRNA construct (to target gene of interest in long-term experiments ) are important gene expresion research tool. However, generation of RNAi stably-expressing cancer cell lines can be very laborious, expensive, and time-consuming. Altogen Labs offer generation of clonal stable knockdown cell lines based on client individual specifications. Our scientists bring years of extensive cell culture experience and expertise. Service includes:
a) Cloning: plasmid DNA encoding antibiotic-resistant gene, synthesis and cloning of shRNA construct targeting gene of interest into plasmid DNA, final plasmid DNA amplification, Maxi-prep purification.
b) Stable cell line generation: transfection of plasmid DNA (at least 20 ug should be provided by client) into a cell line of choice, drug selection of clonal cells, generation of stably-expressing population of cells, validation of shRNA construct expression and RNAi gene silencing by real-time qRT-PCR (mRNA expression) or Western Blot (protein expression).
Another option is generation of Tetracycline inducible RNAi stably-expressing cell lines.
see also: In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi Gene Knockdown Cell Lines | RNAi Screen Pathway Analysis: siRNA and microRNA | RNAi Manufacturing and CRO Services | PCR Cloning: DNA Vector Construction (shRNA) | Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB | Genome-wide siRNA and microRNA High-throughput Screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA Liposome Encapsulation Service | Transient (siRNA) and Stable (shRNA) Transfection Services