Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- BACTERIA ID 16S SEQUENCING
Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
Over 50 assays for median inhibition concentration determination (IC50).
Cell lines available for IC50 compound in vitro testing:
A549 :: (human lung carcinoma cells)
Astrocytes :: (primary astrocyte cells)
HCN-1A :: (human cortical neuronal cells)
CLBPEC :: (human neuroblastoma cells)
HeLa :: (human cervix adenocarcinoma cells)
HepG2 :: (human hepatocellular carcinoma cells)
Cos-7 :: (monkey kidney cells)
ASMC :: (aortic smooth muscle cells)
DU145 :: (human prostate cancer cells)
AsPC-1 :: (human pancreatic cells)
FaDu :: (human hypopharyngeal carcinoma cells)
Fibroblasts :: (primary fibroblast cells)
HMEC :: (human mammary epithelial cancer cells)
HUVEC :: (umbilical vein endothelial cells)
MDA-MB :: (breast cancer cells)
HCAEC :: (human coronary artery endothelial cells)
HuH-7 :: (human hepatoma cells)
BMS2 :: (bone marrow stromal cells)
Caki-1 :: (renal carcinoma cells)
Calu-6 :: (human renin-expressing cells)
CFPEo :: (human trachea epithelium cells)
CHO :: (hamster ovary epithelial cells)
Chromaffin cells :: (chromaffin cell cultures)
D-407 :: (retinal pigment epithelial cells)
D-451 :: (murine fibroblast cells)
DI-TNC1 :: (rat brain astrocyte cells)
HEK-293 :: (human epithelial kidney cells)
HSC-2 :: (human chondrocyte-like cells)
HT-29 :: (human colorectal adenocarcinoma cells)
HTB :: (human osteosarcoma cells)
KB :: (human mouth epidermal carcinoma cells)
Keratinocyte :: (keratinocyte cells)
L929 :: (murine fibrosarcoma cells)
LNCaP :: (prostate cancer cells)
MA-10 :: (Leydig tumour cells)
MCF-7 :: (human breast cancer cells)
MEF :: (murine embryonic fibroblast cells)
MES :: (mouse embryonic stem cells)
MM39 :: (human tracheal gland cells)
MRC-5 :: (human diploid fibroblast cells)
NIH3T3 :: (mouse fibroblast cells)
NRK :: (normal rat kidney epithelial cells)
P815 :: (murine mastocytoma cells)
PAE :: (porcine aortic endothelial cells)
PANC :: (human pancreatic cancer cells)
PC-12 :: (rat pheochromocytoma cells)
PC-3 :: (human prostate cancer cells)
PGHAM :: (hamster pancreatic carcinoma cells)
PHC :: (primary hepatocarcinoma cells)
PS-1 :: (prostate smooth muscle cells)
RAW-1 :: (murine macrophage cells)
WERI-Rb-1 :: (retinoblastoma cells)
SaOS :: (human osteoblastic cells)
SHEP :: (human neuroblastoma cells)
SKNAS :: (bone marrow neuroblastoma cells)
SK-N-MC :: (neuroblastoma cells)
SW-13 :: (human adrenocortical carcinoma cells)
U87 :: (glioblastoma cells)
VERO :: (African green monkey kidney cells)
3LL :: (murine Lewis lung carcinoma cells)
Standard Service Quote:
Service ID: IC50 In Vitro Compound Screening
Service description: Cell-based assay in 96-well format.
Phenotype: Dose-response inhibition of cancer cell line proliferation
Test performed in following cell lines of interest: HepG2, HEPA and normal human fibroblast cells (3)
Number of compounds: 3
Negative control group: 1
Assay type: Dose-response
Number of each compound concentrations to test: 10
Cell-cased assay sample replicates: Duplicate
Total number of samples (data points): 240
Estimated timeline: 2 weeks
Price: $4,250
see also: Pharmacology and toxicology testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi development: targeted siRNA, qPCR assays | Liposome Encapsulation services: siRNA, miRNA, DNA, proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting Services: technology delevopment & evaluation