Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- BACTERIA ID 16S SEQUENCING
Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
The customer sends the cell line(s) or compound of interest, and company scientists perform an apoptosis cell-based assay (> 70 cancer cell lines are available).
The relative amount of caspase per cell is estimated using luminogenic caspase-3 and caspase-7 substrates in a buffer system optimized for caspase activity, luciferase activity, and cell lysis.
Cells in logarithmic growth are seeded in 96-well plates in 0.2 mL media. Cells are cultured with a single concentration of compound of interest.
Cells are harvested at 6, 12, 24, and 48 hr after addition of compound.
The caspase reagent is added to the cells in the well, resulting in cell lysis, followed by caspase cleavage of the substrate and generation of a “glow-type” luminescent signal (produced by the luciferase reaction).
A luminometer is used to measure luminescence, and the signal generated is proportional to the amount of caspase activity present.
Once we know the details of your project, we can provide you an immediate price quote (contact e-mail: info@altogenlabs.com). Please note that experimental details will help us to provide an accurate quote and timeline estimate.
see also: Pharmacology and toxicology testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi development: targeted siRNA, qPCR assays | Liposome Encapsulation services: siRNA, miRNA, DNA, proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting Services: technology delevopment & evaluation